The Q800R3 Sonicator is a chromatin / DNA shearing system which provides scientists with a reliable, easy-to-use means of achieving narrow DNA fragment bands at a cost which is thousands of dollars less than competing systems. The second generation Q800R3 improves upon the design with enhanced usability and quieter operation than its predecessor. Get the DNA shearing you need with the confidence and quality expected from the Sonicator brand.

Samples can be sheared to a range of fragment sizes (150bp – 3kb). The system is compatible with commercially available sample tubes and is capable of processing up to 18 samples at one time. Multiple tube rack options can accommodate 50ul - 1ml sample volumes using a variety of standard sample tubes.

The Q800R3 is a complete package including a chiller to control temperature, digital operating system with programmable memory, high intensity ultrasonics and sound reducing enclosure.

Q800R System for DNA Shearing

Data Sheet

Illumina Technical Notes

Q800R3 SYSTEM HIGHLIGHTS:

High throughput
Uses standard sample tubes
Two year warranty

PART NO. Q800R3 INCLUDES:

Generator
Converter
Ultrasonic Horn
Rotation System
Chiller
Cables
Tubing
3 tube rack options
2-year warranty

Chromatin Shearing - Sample Protocols

	Mammalian Chromatin	Yeast Chromatin	C. elegans Chromatin
Cell Type:	HEK 293T (2) 10cm dishes 70-80% confluent	Wild type S. pombe cells grown to an OD of 1.3-1.5 in YEA.	C. elegans culture; Nuclear extract 1mg/ml
Total Sample Volume:	300ul	300ul	500ul
Fixation Time:	1% Formaldehyde, 13 min	1% Formaldehyde, 15 min	2% Formaldehyde, 20 min
Sonicator Amplitude Setting:	70%	100%	70%
Sonication Pulse Rate:	15 seconds On, 45 seconds Off	20 seconds On, 40 seconds Off	30 seconds On, 30 seconds Off
Total Sonication On Time:	30 min	30 min	20 min

DNA Shearing - Sample Protocols

	Bacterial Genomic DNA	Human Skeletal Myoblast Genomic DNA
Cell Type / Concentration:	E.coli / 250ng	LHCN-M2 Skeletal Myoblast / 200ng
Sonication Pulse Rate:	15 seconds On, 15 seconds Off	15 seconds On, 15 seconds Off
Total Sample Volume:	200ul in 0.5ml tubes	200ul in 0.5ml tubes
Total Sonication On Time:	As indicated below	As indicated below
Sonicator Amplitude Setting:	20%	20%

ChIP Protocols

DNA Shearing Protocols

Power Rating	750 Watts
Frequency	20 kHz
Programmable Timer	1 second to 10 Hours
Voltage	110V, 50/60Hz
Generator Dimensions	8" W x 15.25" L x 8.5" H
Enclosure Dimensions	11.5" W x 12" L x 20" H
Chiller Dimensions	13" W x 11" L x 13" H
*	220V not available online, contact us for local distributor.

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